SEQanswers

Go Back   SEQanswers > Applications Forums > Sample Prep / Library Generation



Similar Threads
Thread Thread Starter Forum Replies Last Post
Illumina Adapter and Primer preparation S.Iyengar Sample Prep / Library Generation 8 05-30-2019 12:54 PM
adapter ligation - Illumina Celia Sample Prep / Library Generation 5 03-29-2011 12:06 PM
About illumina's adapter sequences Alex.yang Illumina/Solexa 0 08-27-2010 08:56 AM
Custom Illumina adapter synthesis kmcarr Sample Prep / Library Generation 22 04-23-2010 12:32 AM
Custom Illumina adapter synthesis vibseq Illumina/Solexa 1 11-04-2009 06:29 AM

Reply
 
Thread Tools
Old 04-12-2012, 07:53 PM   #1
uberfinch
Junior Member
 
Location: boston

Join Date: Dec 2011
Posts: 8
Default Illumina adapter--what can I dispense with?

Hi folks,


For my project I want to alter the 5' illumina adapter sequence (aka "universal" adapter) to make the 3'-most 11 bases different from the 11 bases on the other side (the symmetry in the two adapters screws up my experiment). Assuming I add a custom primer for read #1, does anyone expect this to be a problem?


Thanks!
uberfinch is offline   Reply With Quote
Old 04-13-2012, 07:35 AM   #2
Cofactor Genomics
Registered Vendor
 
Location: St. Louis

Join Date: Jan 2010
Posts: 52
Default

Dear Uberfinch,
This should not be a problem, unless you will be running paired-end reads. You are unable to use a custom seq.primer on read two since each lane is not addressed separately at that stage.

Best of luck,

Jon
Cofactor Genomics is offline   Reply With Quote
Reply

Thread Tools

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off




All times are GMT -8. The time now is 07:31 PM.


Powered by vBulletin® Version 3.8.9
Copyright ©2000 - 2020, vBulletin Solutions, Inc.
Single Sign On provided by vBSSO