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Old 04-12-2012, 07:53 PM   #1
Junior Member
Location: boston

Join Date: Dec 2011
Posts: 8
Default Illumina adapter--what can I dispense with?

Hi folks,

For my project I want to alter the 5' illumina adapter sequence (aka "universal" adapter) to make the 3'-most 11 bases different from the 11 bases on the other side (the symmetry in the two adapters screws up my experiment). Assuming I add a custom primer for read #1, does anyone expect this to be a problem?

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Old 04-13-2012, 07:35 AM   #2
Cofactor Genomics
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Location: St. Louis

Join Date: Jan 2010
Posts: 52

Dear Uberfinch,
This should not be a problem, unless you will be running paired-end reads. You are unable to use a custom seq.primer on read two since each lane is not addressed separately at that stage.

Best of luck,

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