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Old 10-05-2010, 03:16 AM   #1
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Location: france

Join Date: Oct 2010
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Question sequence capture and 454


I would like to know if someone tested the protocol MYselect sequence enrichichment for targeted sequencing (MYcroarray) and made sequence with the 454?
I want to do sequence capture on 454 but I can't use nimblegen protocol...
Do you know a protocol?
Thank you for advance
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Old 10-06-2010, 02:02 AM   #2
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Location: Amsterdam

Join Date: Nov 2009
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I use Nimblegen so I can not tell if MYselect works, but I checked the manual on their website and I don't see a problem, just make a general library with a NEB (lot cheaper) or ROCHE kit and hybridize according to their protocol.

Or you could use agilent sureselect, but I guess that is not possible if you can not use Nimblegen.
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