Hello, I have several samples (8 samples from 8 sampling sites) to from metagenomic sequencing. These samples are from different temperature (environemntal samples). I would like to find over-abundant genes in highest temperature sample.
However, the R software package such as baySeq/DEseq/DEseq2 must have a replicate at each site in order to detect the over-abundant genes and get the P value. Which means that I have to sequence twice for each sample, but I didn't. I only have 8 sequencing datasets by far
Any one knows any package does not require replicates?
Thanks,
Ben
However, the R software package such as baySeq/DEseq/DEseq2 must have a replicate at each site in order to detect the over-abundant genes and get the P value. Which means that I have to sequence twice for each sample, but I didn't. I only have 8 sequencing datasets by far
Any one knows any package does not require replicates?
Thanks,
Ben
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