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  • query on various coverage terms

    Hi ,

    I am quite confused by various terms of 'coverage'. Could i please know what actually does Coverage, Base coverage,coverage of the contig, Depth of the coverage, read depth coverage, Sequencing depth means.

    Also from the velvet assemblies i found that the coverage is Kmer coverage.
    For eg:

    >NODE_6_length_310_cov_23.780645

    From the manual i calculated by substituting in the formula: C= (Ck*L)/(L-k+1)
    and obtained the 'C' value and found the average 'C'- value to be >1200. Is this feasible?


    Moreover, How could i know the reference genome size?

    Thank you for your answers.

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