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Old 01-17-2019, 01:50 AM   #1
Iikki
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Location: Helsinki

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Default Single-stranded DNA library preparation?

Hi!

Does anyone here have experience with single-stranded library preparation of genomic DNA?

We have recently whole genome sequenced FFPE-derived DNA with "standard" double-stranded preparation methods, and the data coverage has been quite poor (due to low yield and lack of complexity issues). I have understood that ss-methods have been widely used for sequencing of ancient DNA, and also that they might outperform ds-methods when sequencing FFPE-derived DNA. E.g. https://academic.oup.com/nar/article/45/10/e79/2948436. Can anyone confirm that this is the case? Are there some specific issues with this kind of prep?

Thanks in advance.
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Old 01-17-2019, 07:12 AM   #2
BioDynami
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This may be a better solution for your issue:

http://seqanswers.com/forums/showthread.php?t=82514

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