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Old 07-05-2016, 10:37 AM   #1
shldbcrzy1
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Location: NYC

Join Date: Apr 2016
Posts: 6
Default Primers for immunoprecipitated DNA using ab8580 antibody

I am setting up ChiP seq in the lab and am trying to use a widely popular antibody for chip - ab8580 histone antibody to standardize every step. After I crosslink and sonicate samples using covaris, I immunoprecipitate. Following ChIP i want to run a qPCR to test for antibody enrichment. I have sequence for the primers used from the company for active (GAPDH, PRPL30) and inactive genes (MYO-D, AFM), but these sequences are for human samples and my samples are mouse. how should i align these sequences for my samples ?

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