This seems like a dumb question, but I haven't been able to figure it out.
I sequenced hemoglobin genes using primers I found online/literature. I found the mutation I was looking for, but I've tried locating the primer annealing site on NCBI and I can't figure out where it would bind.
The gene is HBA2. The primers I used for exon 3 (where the mutation is) were:
F: gaggatcacgcgggttg
R: caggaaacagctatgacc (M13 reverse primer)
Any routes besides NCBI people can suggest? I have the sequencing file that shows the sequencing starting... but I don't know if that really shows where the primer annealed.
I sequenced hemoglobin genes using primers I found online/literature. I found the mutation I was looking for, but I've tried locating the primer annealing site on NCBI and I can't figure out where it would bind.
The gene is HBA2. The primers I used for exon 3 (where the mutation is) were:
F: gaggatcacgcgggttg
R: caggaaacagctatgacc (M13 reverse primer)
Any routes besides NCBI people can suggest? I have the sequencing file that shows the sequencing starting... but I don't know if that really shows where the primer annealed.
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