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I have a small query regarding the whole genome re-sequencing of a human genome using SOLiD system from ABI. We have very little amount of DNA and we are able to only make 2 mate pair libraries form it(25 microgram each). Our main interest is to detect genetic variations. We are planning to run 4 slides from one library,pulling it form 8 ePCRs(we will club 2 ePCR products to make one slide) so that we utilize maximum number of beads. We are expecting 8x-10x coverage after running both the libraries. Now I would like to know, is is sufficient coverage to determine true variants and other variation like indel? If it is sufficient coverage what is suggested threshold to call SNPs?
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Proteins are often described as the workhorses of the cell, and identifying their sequences is key to understanding their role in biological processes and disease. Currently, the most common technique used to determine protein sequences is mass spectrometry. While still a valuable tool, mass spectrometry faces several limitations and requires a highly experienced scientist familiar with the equipment to operate it. Additionally, other proteomic methods, like affinity assays, are constrained...04-04-2024, 04:25 PM -
Despite advancements in sequencing platforms and related sample preparation technologies, certain sample types continue to present significant challenges that can compromise sequencing results. Pedro Echave, Senior Manager of the Global Business Segment at Revvity, explained that the success of a sequencing experiment ultimately depends on the amount and integrity of the nucleic acid template (RNA or DNA) obtained from a sample. “The better the quality of the nucleic acid isolated...03-22-2024, 06:39 AM