Hi all,
I'm doing tissue ChIP seq using human liver tissue both in tumor and adjacent non tumor. I found that the liver tumor tissue always with so much fat/oil droplet and it seriously affect my ChIP so can't get enough DNA after pull down.
Anyone can give me suggestions on how to deal with this problem? The tissues' weight always below 0.2g, I think trim the fat before ChIP not feasible as the fat stay inside the cells.
And we interest in some TFs may not very abundant though.
Thanks in advance!
I'm doing tissue ChIP seq using human liver tissue both in tumor and adjacent non tumor. I found that the liver tumor tissue always with so much fat/oil droplet and it seriously affect my ChIP so can't get enough DNA after pull down.
Anyone can give me suggestions on how to deal with this problem? The tissues' weight always below 0.2g, I think trim the fat before ChIP not feasible as the fat stay inside the cells.
And we interest in some TFs may not very abundant though.
Thanks in advance!
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