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Old 11-22-2011, 02:13 PM   #1
Location: ND, USA

Join Date: Oct 2011
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Default How can I estimate overall coverage against a reference database?

I'm doing de novo assembly on RNA-seq data and I do a stand-alone BLAST against a reference transcriptome. I want to know how much of my assembly aligned, preferably as a percentage so I can estimate quality and have simple statistics to present along with the usual average and median contig lengths-

Velvet-Oases 6.3%
Trinity 7.9%

etc. Is there a tool out there that will estimate how much of a reference database I have covered? Essentially I could add up the lengths of each aligned transcript and divide by the length of all the reference transcripts....not sure if this makes sense, but I could use some advice.
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Old 11-22-2011, 05:01 PM   #2
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BEDTools has a coverage function. It will tell you how many bases are covered at each depth.
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