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Hi everyone,
I have some exome data generated on an Illumina HiSeq2000 by Otogenetics. The exome capturing was done with Nimblegen v2.
I've generated some FASTQC data and I notice something strange for the quality of the 5th base in my read. The quality at that position is lower compared with the neighborhood based. I've added the image (left). It's not looking the same as our other exome data (same company, same machine, same kit, same batch). I've added a 'normal' FASTQC image as well (right). Did someone already observe the same pattern? And what would this mean?
I have some exome data generated on an Illumina HiSeq2000 by Otogenetics. The exome capturing was done with Nimblegen v2.
I've generated some FASTQC data and I notice something strange for the quality of the 5th base in my read. The quality at that position is lower compared with the neighborhood based. I've added the image (left). It's not looking the same as our other exome data (same company, same machine, same kit, same batch). I've added a 'normal' FASTQC image as well (right). Did someone already observe the same pattern? And what would this mean?