Yeah, we were quite surprised when I spoke to them and discovered this. In the future we might ask them to use all and increase the cycles to 14 or 15. That would explain A LOT of reasons why we are struggling with our samples (RNA, not so much for DNA).
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Hi jteeee2,
I just wanted to check back in with you and say thank you. We did some testing in the lab and both the primers / Taq you recommended appeared to work well. We submitted that to our Sequencing CORE and we were able to save over 2/3's of those 68 samples. That a huge relief!!! So instead of all 4 out of 72, we now have material for 49. We have a plan to save those other 23 samples and we are asking them to increase the cycles from 12 for future RNA/FFPE runs.
Thank you so much.
Sue
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Proteins are often described as the workhorses of the cell, and identifying their sequences is key to understanding their role in biological processes and disease. Currently, the most common technique used to determine protein sequences is mass spectrometry. While still a valuable tool, mass spectrometry faces several limitations and requires a highly experienced scientist familiar with the equipment to operate it. Additionally, other proteomic methods, like affinity assays, are constrained...-
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