If we want to get from the MP orientation to the PE orientation (by ourselves), is it correct to do the complement of the actual sequence line while the quality (orientation) remains the same? I just want to be sure...
<---- ----> orientation
@ID 1:N:0
CAGCTC
+
??A??B
@ID 2:N:0:
AGGACA
+
????AB
----> <---- orientation
@ID 1:N:0
GTCGAG
+
??A??B
@ID 2:N:0:
TCCTGT
+
????AB
Thanks!
<---- ----> orientation
@ID 1:N:0
CAGCTC
+
??A??B
@ID 2:N:0:
AGGACA
+
????AB
----> <---- orientation
@ID 1:N:0
GTCGAG
+
??A??B
@ID 2:N:0:
TCCTGT
+
????AB
Thanks!
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