Hi All,
I have replicateA.bam and replicateB.bam. Then I use samtools merge replicateA.bam and replicateB.bam. After that, I use cufflink to do the fpkm calculation. Will Fpkm from this result double compared to caluculatation one by one? Maybe because the later one have less reads? The merge one have double reads?
Thank you for your help.
I have replicateA.bam and replicateB.bam. Then I use samtools merge replicateA.bam and replicateB.bam. After that, I use cufflink to do the fpkm calculation. Will Fpkm from this result double compared to caluculatation one by one? Maybe because the later one have less reads? The merge one have double reads?
Thank you for your help.
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