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  • Comparison of 454, Illumina & Ion Torrent

    Hello,

    Does anybody have an actual overview of the pros and cons of these sequecing methods. I have to give a presentation on this issue, but they improve so quickly, that i cant find the latest comparsion of this 3 methods.

    Thank you for your help.

    Greetings,
    GlobalFighter

  • #2
    Performance comparison of benchtop high-throughput sequencing platforms.

    There is a bunch of net stuff here and elsewhere on critiques of the paper (e.g. mine, which has some links to others). Perhaps the most serious issue is that the Ion protocols in particular are much newer than then kits used in the paper, so results should be better on Ion than presented -- but how much better in a typical lab's hands still remains to be established (and by then the kits will have changed again! )

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