Hi, I am very novice about statistics and data analysis. Recently we have done some RNA sequencing with RNA-tag sequence where we pool many samples in tube for library preperation. We did Hiseq 2500 but after demultiplixing we see huge difference between biological replicate. One experiment looks like this COntrol: A- 1miilion, B-5million, c 0.8 million Treatment: A-3 million, B- 5 million, C 12 million. In a word there is a huge depth variation among biological replicate, i am working with bacteria. Can I use this data for differential expression or should re do the sample. Please help a poor phd!!!!
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The field of epigenetics has traditionally concentrated more on DNA and how changes like methylation and phosphorylation of histones impact gene expression and regulation. However, our increased understanding of RNA modifications and their importance in cellular processes has led to a rise in epitranscriptomics research. “Epitranscriptomics brings together the concepts of epigenetics and gene expression,” explained Adrien Leger, PhD, Principal Research Scientist...-
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Proteins are often described as the workhorses of the cell, and identifying their sequences is key to understanding their role in biological processes and disease. Currently, the most common technique used to determine protein sequences is mass spectrometry. While still a valuable tool, mass spectrometry faces several limitations and requires a highly experienced scientist familiar with the equipment to operate it. Additionally, other proteomic methods, like affinity assays, are constrained...-
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