Seqanswers Leaderboard Ad

Collapse

Announcement

Collapse
No announcement yet.
X
 
  • Filter
  • Time
  • Show
Clear All
new posts

  • any recommandation on variant caller for SOLiD data ?

    I want to call rare variants (SNP and short indel) from pooled sample. I used SHRiMP2 to map color-space reads and got BAM file. The questions are:

    1, What variant caller programs are recommanded for SOLiD data?

    2, When general variant caller are used upon SOLiD data, do they utilize the information of CS, CQ tag in the BAM file? And is the information important for SOLiD variant calling?

    3, I have tried to use Bambino but I can't tell whether the result is good or not. Does anyone tell me about Bambino?

  • #2
    Originally posted by pandafengye View Post
    I want to call rare variants (SNP and short indel) from pooled sample. I used SHRiMP2 to map color-space reads and got BAM file. The questions are:

    1, What variant caller programs are recommanded for SOLiD data?
    Since you're using SHRiMP2, i guess you've tried VARiD?

    Comment

    Latest Articles

    Collapse

    • seqadmin
      Essential Discoveries and Tools in Epitranscriptomics
      by seqadmin




      The field of epigenetics has traditionally concentrated more on DNA and how changes like methylation and phosphorylation of histones impact gene expression and regulation. However, our increased understanding of RNA modifications and their importance in cellular processes has led to a rise in epitranscriptomics research. “Epitranscriptomics brings together the concepts of epigenetics and gene expression,” explained Adrien Leger, PhD, Principal Research Scientist...
      Yesterday, 07:01 AM
    • seqadmin
      Current Approaches to Protein Sequencing
      by seqadmin


      Proteins are often described as the workhorses of the cell, and identifying their sequences is key to understanding their role in biological processes and disease. Currently, the most common technique used to determine protein sequences is mass spectrometry. While still a valuable tool, mass spectrometry faces several limitations and requires a highly experienced scientist familiar with the equipment to operate it. Additionally, other proteomic methods, like affinity assays, are constrained...
      04-04-2024, 04:25 PM

    ad_right_rmr

    Collapse

    News

    Collapse

    Topics Statistics Last Post
    Started by seqadmin, 04-11-2024, 12:08 PM
    0 responses
    58 views
    0 likes
    Last Post seqadmin  
    Started by seqadmin, 04-10-2024, 10:19 PM
    0 responses
    53 views
    0 likes
    Last Post seqadmin  
    Started by seqadmin, 04-10-2024, 09:21 AM
    0 responses
    45 views
    0 likes
    Last Post seqadmin  
    Started by seqadmin, 04-04-2024, 09:00 AM
    0 responses
    55 views
    0 likes
    Last Post seqadmin  
    Working...
    X