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  • #1

    Covaris RRL approach

    Hi All,

    I am hoping to use Covaris for fragmenting DNA that is already size selected to fragment size between 2kn and 3kb (using a similar protocol to the Turkey SNP RRL paper http://www.biomedcentral.com/1471-2164/10/479)..

    I understand that parameters are nicely worked out for fragmenting whole genomic DNA, but I have seen less about pre-fragmented stuff..

    I'll end up doing the experiment to determine the optimal conditions, but it would be helpful to know where to start..
    Tags: covaris, illumina, reduced representation
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