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Hello.I have two samples and compare their AS genes.I should analysis one gene that has different events？ Or one gene in 1 sample don't occur AS but in 2 sample occur AS ?
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If you want optimal alternative splicing analysis, you should use BBMap, which has the best alternative splicing recognition, being purely de-novo.
For mammalian RNA-seq, I suggest the flag 'maxindel=200000'. The actual value is highly dependent on the organism(s) you are sequencing.
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The sequencing world is rapidly changing due to declining costs, enhanced accuracies, and the advent of newer, cutting-edge instruments. Equally important to these developments are improvements in sequencing analysis, a process that converts vast amounts of raw data into a comprehensible and meaningful form. This complex task requires expertise and the right analysis tools. In this article, we highlight the progress and innovation in sequencing analysis by reviewing several of the...05-06-2024, 07:48 AM -
The field of epigenetics has traditionally concentrated more on DNA and how changes like methylation and phosphorylation of histones impact gene expression and regulation. However, our increased understanding of RNA modifications and their importance in cellular processes has led to a rise in epitranscriptomics research. “Epitranscriptomics brings together the concepts of epigenetics and gene expression,” explained Adrien Leger, PhD, Principal Research Scientist...04-22-2024, 07:01 AM
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