Hello
I need work with Chip-seq of 3 conditions (WT, C and V). It’s single-end.
When I saw the data, questions born:
1. Each sample has duplicate, but each duplicate has 4 fastq files corresponding to “lanes” of the sequencing. That’s mean that the 4 files correspond to one sample? If so... Would I join the 4 fastq files for work with them as one sample?
2. Other sequencing data are present, with names WTIN, CIN and VIN (each one with 4 fastq files). The text file that I received says: “Each experiment was performed in duplicate, the sequencing was through NextSeq with a 76ntSR reading format, single entry income for each sample marked as WTIN, CIN and VIN.” Someone known whats that mean?
Thanks to everyone!
I need work with Chip-seq of 3 conditions (WT, C and V). It’s single-end.
When I saw the data, questions born:
1. Each sample has duplicate, but each duplicate has 4 fastq files corresponding to “lanes” of the sequencing. That’s mean that the 4 files correspond to one sample? If so... Would I join the 4 fastq files for work with them as one sample?
2. Other sequencing data are present, with names WTIN, CIN and VIN (each one with 4 fastq files). The text file that I received says: “Each experiment was performed in duplicate, the sequencing was through NextSeq with a 76ntSR reading format, single entry income for each sample marked as WTIN, CIN and VIN.” Someone known whats that mean?
Thanks to everyone!
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