Hi all,
I use tophat to analyze one dataset with 27bp length, and I am confused about the coverage.wig file it generated.
Here is one example:
chr1 11116146 11116147 47
chr1 11116147 11116149 45
chr1 11116149 11116150 42
chr1 11116150 11116151 40
chr1 11116151 11116152 5
chr1 11116152 11116154 4
chr1 11116154 11116657 0
chr1 11116657 11116658 3
chr1 11116658 11116659 4
Does the third column stand for the number of reads that cover the site ?
So does it mean there are 47 reads in 11116147 and 11116146 respectively ?
But my reads is only 27bp, so the max number of reads coverage at one site should be < 27, I am confused why there are some sites that the number of reads coverage is > 27.
Does anyone have some ideas ?
I use tophat to analyze one dataset with 27bp length, and I am confused about the coverage.wig file it generated.
Here is one example:
chr1 11116146 11116147 47
chr1 11116147 11116149 45
chr1 11116149 11116150 42
chr1 11116150 11116151 40
chr1 11116151 11116152 5
chr1 11116152 11116154 4
chr1 11116154 11116657 0
chr1 11116657 11116658 3
chr1 11116658 11116659 4
Does the third column stand for the number of reads that cover the site ?
So does it mean there are 47 reads in 11116147 and 11116146 respectively ?
But my reads is only 27bp, so the max number of reads coverage at one site should be < 27, I am confused why there are some sites that the number of reads coverage is > 27.
Does anyone have some ideas ?
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