Hello,
Where to find the exact oligonucleotide sequence of primers for (Read1, Index1 and Read2) which are in the Miseq Kits V2.
I tried to find this information in different places but without a result.
I use custom protocol for library preparation. I use following adapters P5, P7
P5 ACACTCTTTCCCTACACGACGCTCTTCCGATCT
P7 GTGACTGGAGTTCAGACGTGTGCTCTTCCGATCT
My question is do I have to use custom primers for sequencing or I can use those from the Miseq V2 kit.
Thanks in advance for your help
Where to find the exact oligonucleotide sequence of primers for (Read1, Index1 and Read2) which are in the Miseq Kits V2.
I tried to find this information in different places but without a result.
I use custom protocol for library preparation. I use following adapters P5, P7
P5 ACACTCTTTCCCTACACGACGCTCTTCCGATCT
P7 GTGACTGGAGTTCAGACGTGTGCTCTTCCGATCT
My question is do I have to use custom primers for sequencing or I can use those from the Miseq V2 kit.
Thanks in advance for your help
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