Seqanswers Leaderboard Ad

Collapse

Announcement

Collapse
No announcement yet.
X
 
  • Filter
  • Time
  • Show
Clear All
new posts

  • Breakdancer_max output is empty

    HI ,

    I am trying to use breakdancer for the first time and struggling a bit with the command lines
    this is the version I have installed
    breakdancer-1.1_2011_02_21

    I have created my config file using
    /breakdancer-1.1_2011_02_21/perl/bam2cfg.pl file#1.bam > file#1.bam.BD_config
    this is the file
    readgroup:NA platform:illumina map:file1#1.bam readlen:75.00 lib:NA num:9997 lower:32.49 upper:392.41 me
    an:170.75 std:44.51 SWnormality:-54.43 exe:samtools view

    hwne I run BreakdanceMax I create only the header of a file
    /breakdancer-1.1_2011_02_21/cpp/breakdancer_max -t file#1.bam.BD_config >test.out

    this is the test.out
    #Command: /breakdancer-1.1_2011_02_21/cpp/breakdancer_max -t file#1.bam.BD_config
    #Library Statistics:
    #file#1.bam mean:170.75 std:44.51 uppercutoff:392.41 lowercutoff:32.49 readlen:75 library:NA reflen:303
    9910766 seqcov:0.745619 phycov:0.848763 32:61859
    #Chr1 Pos1 Orientation1 Chr2 Pos2 Orientation2 Type Size Score num_Reads num_Reads_lib file#1.bam

    any clues/tips?
    thanks a lot

  • #2
    Did you figure this out? And if you did, could you share the solution?
    I also encountered this problem.
    Thanks a lot!

    Comment


    • #3
      Do you have the index bam file corresponding to your bam in the same directory?
      For troubleshooting, perl version of BreakDancerMax is helpful. Error message are more clear than C version.
      Hope this help.

      Comment


      • #4
        hi,jeny
        I do have the index file.
        I may try perl version.
        Thanks.

        ------------

        I have tried the BreakDancerMax.pl. Unfortunately, the output was empty expect #header, too. And there was not any error information.

        Thanks all the same.
        Last edited by liying; 12-26-2011, 10:31 PM.

        Comment


        • #5
          have u guys figured it out? I just ran into the same problem

          Comment

          Latest Articles

          Collapse

          • seqadmin
            Recent Advances in Sequencing Analysis Tools
            by seqadmin


            The sequencing world is rapidly changing due to declining costs, enhanced accuracies, and the advent of newer, cutting-edge instruments. Equally important to these developments are improvements in sequencing analysis, a process that converts vast amounts of raw data into a comprehensible and meaningful form. This complex task requires expertise and the right analysis tools. In this article, we highlight the progress and innovation in sequencing analysis by reviewing several of the...
            05-06-2024, 07:48 AM
          • seqadmin
            Essential Discoveries and Tools in Epitranscriptomics
            by seqadmin




            The field of epigenetics has traditionally concentrated more on DNA and how changes like methylation and phosphorylation of histones impact gene expression and regulation. However, our increased understanding of RNA modifications and their importance in cellular processes has led to a rise in epitranscriptomics research. “Epitranscriptomics brings together the concepts of epigenetics and gene expression,” explained Adrien Leger, PhD, Principal Research Scientist...
            04-22-2024, 07:01 AM

          ad_right_rmr

          Collapse

          News

          Collapse

          Topics Statistics Last Post
          Started by seqadmin, 05-10-2024, 06:35 AM
          0 responses
          20 views
          0 likes
          Last Post seqadmin  
          Started by seqadmin, 05-09-2024, 02:46 PM
          0 responses
          25 views
          0 likes
          Last Post seqadmin  
          Started by seqadmin, 05-07-2024, 06:57 AM
          0 responses
          21 views
          0 likes
          Last Post seqadmin  
          Started by seqadmin, 05-06-2024, 07:17 AM
          0 responses
          21 views
          0 likes
          Last Post seqadmin  
          Working...
          X