I have an e. coli genome and I want to know which genes are paralogs so I can investigate how RNA-seq reads are mapping to multiple positions. What I would like to do is get a full list of paralogs within my e.coli genome. I could blast the genome against itself and write some scripts to sort and select potential paralogs, but it seems like a problem that many people have likely encountered in the past and I don't want to reinvent the wheel if I don't have to. Is there a tool out there that can do this sort of thing already? Thanks very much!
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by seqadmin
The field of epigenetics has traditionally concentrated more on DNA and how changes like methylation and phosphorylation of histones impact gene expression and regulation. However, our increased understanding of RNA modifications and their importance in cellular processes has led to a rise in epitranscriptomics research. “Epitranscriptomics brings together the concepts of epigenetics and gene expression,” explained Adrien Leger, PhD, Principal Research Scientist...-
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Proteins are often described as the workhorses of the cell, and identifying their sequences is key to understanding their role in biological processes and disease. Currently, the most common technique used to determine protein sequences is mass spectrometry. While still a valuable tool, mass spectrometry faces several limitations and requires a highly experienced scientist familiar with the equipment to operate it. Additionally, other proteomic methods, like affinity assays, are constrained...-
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