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Old 02-12-2013, 02:21 PM   #1
mariebreen
Junior Member
 
Location: Iowa City

Join Date: Feb 2013
Posts: 3
Default Methyl-Seq Questions

Hi all,

I am trying to set up a global methylation project using Agilent's Methyl-Seq system but I'm having some trouble analyzing my sequencing results.

I was hoping those out there who are also attempting Methyl-Seq could give me their opinion on a few steps that I feel could be the problem:

How many samples to multiplex? I am currently trying 4.
How much PhiX DNA to spike in to account for the sequence bias? I have been using 1%.

Any help would be greatly appreciated,
Marie
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