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10-30-2012, 06:09 AM
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#1 |
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Junior Member
Location: Zürich, Switzerland Join Date: Oct 2012
Posts: 1
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input DNA for Illumina´s Nextera XT sample prep kit
Hi All-
I am generating PCR product of DNA sampled from water (environmental DNA). The amplicons range in size from 650-790 bp. This is the input DNA I would like to use for the Nextera XT sample prep kit from Illumina. I have asked their tech support with out much help on this topic... If I have a nice clean and tight PCR band on a gel, is there any reason why one can not use exo and sap to "clean" the PCRs before diluting the DNA to 1 ng/uL? I have over 600 PCRs and doing gel extraction or even a magnetic bead clean up is too much time and money. Thanks!! alpinedna |
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11-05-2012, 06:29 AM
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#2 | |
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Member
Location: USA Join Date: Nov 2011
Posts: 17
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PCR clean-up for sequencing
Quote:
Given how much money you are about to spend sequencing these samples, and the potential precious nature of them, I would not risk it. Do the magnetic bead clean-up! If you do it in a 96-well plate it shouldn't take too much time at all. A simple clean-up with 0.6x bead volume would suffice. |
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