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Old 12-06-2011, 11:54 AM   #1
Giorgio C
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Location: ITALY

Join Date: Oct 2010
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Default miRNA's expression normalization

Hi everybody,

i have two set of miRNAs: A|B : The first has obtained 1/3 of the total sequences of the second. How do i need to consider the level expression ?

For example if i have 2000 reads for a particular miRNA X in the set A and 1000 in the B it means that miRNA X is about 2x expressed in A respect to B?

If is it like i think i should have a normalization....

What you suggest ?? Is there any way, script, tools that may help ???

Thank you very much...
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