I am planning to use CORTEX to assemble 454 data and I want to use the cut-homopolymer option but I am having trouble deciding at what size homopolymers are a problem for 454. Seems to me like it would somewhere in the 4-6 base range, however I would like to disrupt my reads as little as possible.
Does anyone has some insight into this? Has anyone looked at error rates as a function of homopolymer length in 454?
Thanks,
David
Does anyone has some insight into this? Has anyone looked at error rates as a function of homopolymer length in 454?
Thanks,
David
Comment