Illumina's normalization beads have formamide, are very stinky, must be used in a hood and use NaOH to render the DNA single stranded. I looked into alternative bead normalization products (one from Qiagen and one from MagBio) and they do not seem to have any of the nasty chemicals in them. I think they are just concentrated AMPure with PEG and NaCl.
Has anyone figured out what is in Illumina's beads? Does anyone have experience with the commercial bead normalization products other than Illumina's or have made your own cocktail? Any ideas on why the ingredients are different or why one would be better than the other?
I am asking because library qPCR takes soooo long when processing high throughput!
Has anyone figured out what is in Illumina's beads? Does anyone have experience with the commercial bead normalization products other than Illumina's or have made your own cocktail? Any ideas on why the ingredients are different or why one would be better than the other?
I am asking because library qPCR takes soooo long when processing high throughput!
Comment