Hi
I just saw the new NEXTflex RNA-Seq kit. They have this interesting "molecular indice" that would label each dsDNA molecule.
When I took a closer look, I can see that this "indice" is most likely to be a pool of barcode adapters (as in Craig et al 2004 Nat Methods). I think they also have the 3' end matches the truseq adapter. Unlike the truseq adapter, one aditional barcode could be placed before the T overhang. Nextflex claims to have 9,216 barcode in each adapter. That is 4x4x4x4x4x3x3. They could synthesize/anneal 9,216 adapters but I think it is more reasonable to use 7 random nucleotide (during oligo synthesis) barcode before the T overhang. In order to make the adapter, they must be able to anneal it to a complementary oligo. I assume they could use one (or a few) with deoxyinosine.
Anyone has more information? I am just guessing, and could be completely wrong.
Cheers
silin
I just saw the new NEXTflex RNA-Seq kit. They have this interesting "molecular indice" that would label each dsDNA molecule.
When I took a closer look, I can see that this "indice" is most likely to be a pool of barcode adapters (as in Craig et al 2004 Nat Methods). I think they also have the 3' end matches the truseq adapter. Unlike the truseq adapter, one aditional barcode could be placed before the T overhang. Nextflex claims to have 9,216 barcode in each adapter. That is 4x4x4x4x4x3x3. They could synthesize/anneal 9,216 adapters but I think it is more reasonable to use 7 random nucleotide (during oligo synthesis) barcode before the T overhang. In order to make the adapter, they must be able to anneal it to a complementary oligo. I assume they could use one (or a few) with deoxyinosine.
Anyone has more information? I am just guessing, and could be completely wrong.
Cheers
silin
Comment