Dear all,
this is my first post and generally will show you how fresh I am
Till now I have prepared few WES sequencing using NextSeq machine and then fastq/ Isaac Enrichment workflow. But this is not my problem right now.
Last time I prepared an additional group of 4 samples (bacterial genomes, estimated size near 4M bp). I used Nextera XT kit for libraries preparation, and do some calculations to add only 1% of all libraries for human WES sequencing in highoutput mode.
And there was a mistake (happens) and my bacterial genomes have much more data than I thought and design. Every one after fastq gives me near 5Gb of data. I imported them do BaseSpace (cloud), (cause I have BSonsite) because I wanted to use free apps there for de novo assembly Like (Velvet or SPades) unfortunatelly these apps in BS are not able to use too much data (for Velvet coverage below 100, and for SPades below 1G of bases).
Any suggestion?
I performed analysis used another app (from DNAStar) and two of my bacterial DNA are similar to Bacillus cereus.
In that case I wish to perform resequencing analysis too. What can you suggest to me?
Regards,
L.
this is my first post and generally will show you how fresh I am
Till now I have prepared few WES sequencing using NextSeq machine and then fastq/ Isaac Enrichment workflow. But this is not my problem right now.
Last time I prepared an additional group of 4 samples (bacterial genomes, estimated size near 4M bp). I used Nextera XT kit for libraries preparation, and do some calculations to add only 1% of all libraries for human WES sequencing in highoutput mode.
And there was a mistake (happens) and my bacterial genomes have much more data than I thought and design. Every one after fastq gives me near 5Gb of data. I imported them do BaseSpace (cloud), (cause I have BSonsite) because I wanted to use free apps there for de novo assembly Like (Velvet or SPades) unfortunatelly these apps in BS are not able to use too much data (for Velvet coverage below 100, and for SPades below 1G of bases).
Any suggestion?
I performed analysis used another app (from DNAStar) and two of my bacterial DNA are similar to Bacillus cereus.
In that case I wish to perform resequencing analysis too. What can you suggest to me?
Regards,
L.
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