Hey,
just wondering if anyone has tried omiting the gel-size selection following Illumina adaptor ligation prior to commemcing the LM-PCR step in the NimbleGen in-solution Exon capture protocol?
If so does the residual adaptor dimers have any marked effect on the downstream data?
Cheers,
Mitch.
just wondering if anyone has tried omiting the gel-size selection following Illumina adaptor ligation prior to commemcing the LM-PCR step in the NimbleGen in-solution Exon capture protocol?
If so does the residual adaptor dimers have any marked effect on the downstream data?
Cheers,
Mitch.
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