A collaborator has prepared a custom library for our sequencing by amplifying with the following primers.
Multiplexing PCR Primer 1.0:
AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACGCTCTTCCGATCT
Multiplexing PCR Primer 2.0:
GTGACTGGAGTTCAGACGTGTGCTCTTCCGATCT
I told them the library was not yet ready to sequence because Multiplexing PCR Primer 2.0 does not have a flowcell sequence (which it definitely doesn't). Am I correct that the library is not yet ready to sequence?
I recognize the primer's name as belonging to an older version of the Illumina-supplied oligo scheme, but am not sure of how the final library was created in that scheme.
Can someone who used the older scheme let us know if I know what I'm talking about?
Thank you!
Multiplexing PCR Primer 1.0:
AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACGCTCTTCCGATCT
Multiplexing PCR Primer 2.0:
GTGACTGGAGTTCAGACGTGTGCTCTTCCGATCT
I told them the library was not yet ready to sequence because Multiplexing PCR Primer 2.0 does not have a flowcell sequence (which it definitely doesn't). Am I correct that the library is not yet ready to sequence?
I recognize the primer's name as belonging to an older version of the Illumina-supplied oligo scheme, but am not sure of how the final library was created in that scheme.
Can someone who used the older scheme let us know if I know what I'm talking about?
Thank you!
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