Seqanswers Leaderboard Ad

Collapse

Announcement

Collapse
No announcement yet.
X
 
  • Filter
  • Time
  • Show
Clear All
new posts

  • creating tab-delimited output from unix

    greetings, i have a large list of taxonomic information from a blast search in a text file (taxa.txt, see below).

    $ head -5 taxa.txt
    Eukaryota;Opisthokonta;Fungi;Ascomycota
    Bacteria;Candidate division OD1;marine metagenome
    Bacteria;Planctomycetes;Phycisphaerae
    Bacteria;Cyanobacteria;Chloroplast;
    Eukaryota;Opisthokonta;Fungi;Ascomycota;

    What i would like to do is create a bar plot of this data in excel or R. To do this, I have been using sort and uniq to tally up each of the hits.

    $sort -u taxa.txt|uniq -c > sorted_taxa.txt
    $head -5 sorted_taxa.txt
    1 Archaea;Euryarchaeota;Halobacteria
    1 Archaea;Euryarchaeota;Thermoplasmata
    1 Archaea;Thaumarchaeota;Marine Group I
    1 Archaea;Thaumarchaeota;Marine Group I
    1 Archaea;Thaumarchaeota;Soil Crenarchaeotic Group(SCG)

    However, when i open this file, and copy and paste the results into excel, it treats each row as one single string. What I would like is for there to be two columns (at least) in output, the column describing the count, and the column describing the taxonomic information. I believe this would be tab-delimited output. Is there a way to tell unix to produce such a result? Thanks,

    -Tony

  • #2
    Hi Tony,

    First thing I noticed is that I think you want to remove the "-u" flag from the sort. The -u flag will remove duplicate lines, so when you do "uniq -c", you will get all 1s.

    Something like this might work:

    Code:
    sort taxa.txt | uniq -c | awk '{$1=$1};1' | awk '{sub(/\ /,"\t")};1' > histo.txt
    The first awk command gets rid of leading, trailing, and extra white spaces. The second awk command replaces the first space with a tab (both commands from awk one-liners).

    Hope that helps,
    Justin

    Comment


    • #3
      Why not open it in Excel (or R or ...) but specify the separators are semi-colons?

      Comment

      Latest Articles

      Collapse

      • seqadmin
        Current Approaches to Protein Sequencing
        by seqadmin


        Proteins are often described as the workhorses of the cell, and identifying their sequences is key to understanding their role in biological processes and disease. Currently, the most common technique used to determine protein sequences is mass spectrometry. While still a valuable tool, mass spectrometry faces several limitations and requires a highly experienced scientist familiar with the equipment to operate it. Additionally, other proteomic methods, like affinity assays, are constrained...
        04-04-2024, 04:25 PM
      • seqadmin
        Strategies for Sequencing Challenging Samples
        by seqadmin


        Despite advancements in sequencing platforms and related sample preparation technologies, certain sample types continue to present significant challenges that can compromise sequencing results. Pedro Echave, Senior Manager of the Global Business Segment at Revvity, explained that the success of a sequencing experiment ultimately depends on the amount and integrity of the nucleic acid template (RNA or DNA) obtained from a sample. “The better the quality of the nucleic acid isolated...
        03-22-2024, 06:39 AM

      ad_right_rmr

      Collapse

      News

      Collapse

      Topics Statistics Last Post
      Started by seqadmin, 04-11-2024, 12:08 PM
      0 responses
      30 views
      0 likes
      Last Post seqadmin  
      Started by seqadmin, 04-10-2024, 10:19 PM
      0 responses
      32 views
      0 likes
      Last Post seqadmin  
      Started by seqadmin, 04-10-2024, 09:21 AM
      0 responses
      28 views
      0 likes
      Last Post seqadmin  
      Started by seqadmin, 04-04-2024, 09:00 AM
      0 responses
      52 views
      0 likes
      Last Post seqadmin  
      Working...
      X