Hi guys,
This may be a really naive question for you, but I have no ChIPseq or bioinformatic experience at all.
I have done chipseq with the same antibody in fly but under two different conditions. For each condition, I've done peak calling (MACS) using input control. Then how do i normalize these two datasets? Apparently one dataset has more reads than the other. i did some literature research. Some people said "using library size". Some other said "using mean coverage". Don't really understand how i could do it. Is there any program i can use?
Thank you so much for great patience and help!!!
This may be a really naive question for you, but I have no ChIPseq or bioinformatic experience at all.
I have done chipseq with the same antibody in fly but under two different conditions. For each condition, I've done peak calling (MACS) using input control. Then how do i normalize these two datasets? Apparently one dataset has more reads than the other. i did some literature research. Some people said "using library size". Some other said "using mean coverage". Don't really understand how i could do it. Is there any program i can use?
Thank you so much for great patience and help!!!
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