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  • ThruPLEX kit: anyone who has some experience?

    Dear all,

    I was trying to figure out what the kit ThruPLEX contains.
    In the US Patent 8,206,913 there are a lot of interesting info about the more recent PicoPLEX kit, so one can find out exactly what each tube contains.

    However, for the ThruPLEX kit (US Patents 7,803,550-8,071,312-8,399,199) the protocols and PCR programs reported in the patent are completely different from the manual you get with the kit.
    How the hairpin oligos work and how they don´t form dimers seems really clear...but I´m deeply confused about the rest!

    Anybody out there who tried to figure out what he/she is doing when adding reagents from all those colourful tubes?
    Thanks!

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