Seqanswers Leaderboard Ad

Collapse

Announcement

Collapse
No announcement yet.
X
 
  • Filter
  • Time
  • Show
Clear All
new posts

  • FastQC, v0.11.5, error: Too many tiles ...

    Ubuntu

    Hi,

    I have FastQC, v0.11.5 and I want to evaluate an Illumina run (pairend). I have reads of 150bp and a average coverage near to 60X.

    When I run FastQC after some time I have this kind of error:

    "Too many tiles (>500) so giving up trying to do per-tile qualities since we're probably parsing the file wrongly"

    I tried to increase the threads with "-t 10" but I still have the same error.

    What I have to do to fix this error?

    Best,
    echo manolis

  • #2
    If this is NovaSeq data then you should upgrade to the latest FastQC (v. 0.11.7).

    Comment


    • #3
      ok thanks. I will try and I will let you know!

      Best

      Comment

      Latest Articles

      Collapse

      • seqadmin
        Current Approaches to Protein Sequencing
        by seqadmin


        Proteins are often described as the workhorses of the cell, and identifying their sequences is key to understanding their role in biological processes and disease. Currently, the most common technique used to determine protein sequences is mass spectrometry. While still a valuable tool, mass spectrometry faces several limitations and requires a highly experienced scientist familiar with the equipment to operate it. Additionally, other proteomic methods, like affinity assays, are constrained...
        04-04-2024, 04:25 PM
      • seqadmin
        Strategies for Sequencing Challenging Samples
        by seqadmin


        Despite advancements in sequencing platforms and related sample preparation technologies, certain sample types continue to present significant challenges that can compromise sequencing results. Pedro Echave, Senior Manager of the Global Business Segment at Revvity, explained that the success of a sequencing experiment ultimately depends on the amount and integrity of the nucleic acid template (RNA or DNA) obtained from a sample. “The better the quality of the nucleic acid isolated...
        03-22-2024, 06:39 AM

      ad_right_rmr

      Collapse

      News

      Collapse

      Topics Statistics Last Post
      Started by seqadmin, 04-11-2024, 12:08 PM
      0 responses
      18 views
      0 likes
      Last Post seqadmin  
      Started by seqadmin, 04-10-2024, 10:19 PM
      0 responses
      22 views
      0 likes
      Last Post seqadmin  
      Started by seqadmin, 04-10-2024, 09:21 AM
      0 responses
      17 views
      0 likes
      Last Post seqadmin  
      Started by seqadmin, 04-04-2024, 09:00 AM
      0 responses
      49 views
      0 likes
      Last Post seqadmin  
      Working...
      X