I'm new in next generation sequencing, and I use Bowtie for the first time. Is there someone that know how to get only the sequences that only have one match? I tried this command.
bowtie hg19 -q input.fastq -m 1 --best --strata --all -S bowtie_out. I also tried without the --all command but both gave equal numbers of hits.
Thanks
bowtie hg19 -q input.fastq -m 1 --best --strata --all -S bowtie_out. I also tried without the --all command but both gave equal numbers of hits.
Thanks
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