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Can any one tell how to set the expected coverege parameter in velvet denovo assembly , if i have the illumina reads of 0.1x cov.
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Take a look at section 5.2 and 5.3 of the velvet manual:
5.2 Choice of a coverage cutoff (14)
5.3 Determining the expected coverage (16)--
Senthil Palanisami -
hi ,thanks for your reply...From the manual it is stated that with reference to the graph the expected cov is calculated as 14x ,so what i understood is we should take the value of the highest peak.am I right ?Comment
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You could always just use "-cov_cutoff auto" (take a look at the manual), but I prefer to avoid magic when possible.
I would make the graph and use it as a starting point, although I recommend then tweaking the parameters and seeing what you get (especially do'able since it is done in the second step, velvetg).--
Senthil PalanisamiComment
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