Epicentre's ScriptMiner Kit has been improved with a streamlined protocol that requires less hands-on time. The ScriptMiner Kit produces nonbarcoded or barcoded Illumina sequencing libraries from small RNA, including miRNA. The kit contains Tobacco Acid Pyrophosphatase (TAP), enabling capture of small 5´-capped RNA and small 5´-triphosphorylated RNA. A unique enzymatic procedure greatly reduces adaptor-dimer formation in the sequencing library.
Seqanswers Leaderboard Ad
Collapse
Announcement
Collapse
No announcement yet.
X
-
Hi,
thanks for the post. I have 2 questions about the new protocol: What is the optimum temperature for the Scriptminer 3'-RNA and the 5'-RNA ligase? I have to do the incubation in the thermocycler as our lab is too cold.
The PCR amplification step states to use 40-45 uL di-tagged cDNA - however, we have only 40uL cDNA in total after the cDNA synthesis. Is that a typo?
thank you,
Latest Articles
Collapse
-
by seqadmin
The field of epigenetics has traditionally concentrated more on DNA and how changes like methylation and phosphorylation of histones impact gene expression and regulation. However, our increased understanding of RNA modifications and their importance in cellular processes has led to a rise in epitranscriptomics research. “Epitranscriptomics brings together the concepts of epigenetics and gene expression,” explained Adrien Leger, PhD, Principal Research Scientist...-
Channel: Articles
Yesterday, 07:01 AM -
-
by seqadmin
Proteins are often described as the workhorses of the cell, and identifying their sequences is key to understanding their role in biological processes and disease. Currently, the most common technique used to determine protein sequences is mass spectrometry. While still a valuable tool, mass spectrometry faces several limitations and requires a highly experienced scientist familiar with the equipment to operate it. Additionally, other proteomic methods, like affinity assays, are constrained...-
Channel: Articles
04-04-2024, 04:25 PM -
ad_right_rmr
Collapse
News
Collapse
Topics | Statistics | Last Post | ||
---|---|---|---|---|
Started by seqadmin, 04-11-2024, 12:08 PM
|
0 responses
58 views
0 likes
|
Last Post
by seqadmin
04-11-2024, 12:08 PM
|
||
Started by seqadmin, 04-10-2024, 10:19 PM
|
0 responses
54 views
0 likes
|
Last Post
by seqadmin
04-10-2024, 10:19 PM
|
||
Started by seqadmin, 04-10-2024, 09:21 AM
|
0 responses
45 views
0 likes
|
Last Post
by seqadmin
04-10-2024, 09:21 AM
|
||
Started by seqadmin, 04-04-2024, 09:00 AM
|
0 responses
55 views
0 likes
|
Last Post
by seqadmin
04-04-2024, 09:00 AM
|
Comment