Last thread on a similar topic was back in 2008 so figured I'd check if anything has changed - to save time whilst performing a genomic library prep (TruSeq) I decided to try a 0.6x AMPure cleanup instead of gel-cutting for size-selection. Resulting libraries are a bit bigger than I was expecting - around 700bp enriched product so insert size of ~600bp! Are fragments this size going to cluster correctly?
I suppose I could burn a MiSeq run to find out before risking them on the HiScan... but any advice appreciated!
I suppose I could burn a MiSeq run to find out before risking them on the HiScan... but any advice appreciated!
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