Brief Communication
Nature Methods 7, 130 - 132 (2010)
Published online: 17 January 2010 | doi:10.1038/nmeth.1417
FRT-seq: amplification-free, strand-specific transcriptome sequencing
Lira Mamanova1,3, Robert M Andrews1,3, Keith D James1, Elizabeth M Sheridan1, Peter D Ellis1, Cordelia F Langford1, Tobias W B Ost2, John E Collins1 & Daniel J Turner1
Abstract
We report an alternative approach to transcriptome sequencing for the Illumina Genome Analyzer, in which the reverse transcription reaction takes place on the flowcell. No amplification is performed during the library preparation, so PCR biases and duplicates are avoided, and because the template is poly(A)+ RNA rather than cDNA, the resulting sequences are necessarily strand-specific. The method is compatible with paired- or single-end sequencing.
Nature Methods 7, 130 - 132 (2010)
Published online: 17 January 2010 | doi:10.1038/nmeth.1417
FRT-seq: amplification-free, strand-specific transcriptome sequencing
Lira Mamanova1,3, Robert M Andrews1,3, Keith D James1, Elizabeth M Sheridan1, Peter D Ellis1, Cordelia F Langford1, Tobias W B Ost2, John E Collins1 & Daniel J Turner1
Abstract
We report an alternative approach to transcriptome sequencing for the Illumina Genome Analyzer, in which the reverse transcription reaction takes place on the flowcell. No amplification is performed during the library preparation, so PCR biases and duplicates are avoided, and because the template is poly(A)+ RNA rather than cDNA, the resulting sequences are necessarily strand-specific. The method is compatible with paired- or single-end sequencing.