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Old 03-04-2016, 11:04 AM   #1
Location: cleveland

Join Date: Dec 2015
Posts: 26
Default How to interpret this HS DNA library profile

Please see attached PDF and give your feedback. Are these good quality libraries for RNA seq.

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File Type: pdf HS DNA library.pdf (1.77 MB, 47 views)
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Old 03-04-2016, 01:42 PM   #2
Jafar Jabbari
Location: Melbourne

Join Date: Jan 2013
Posts: 1,232

They do not seem to be good libraries for sequencing. Fragment size distributions are different among libraries indicating issues with library prep which will affect downstream sequencing and analysis negatively. Four of the libraries have high level of prime/adapter-dimers. Libraries are also overloaded for HS Chip.
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