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  • Make Multi-fasta with annotations

    Hi everyone

    Im ending my work and i have a problem, how can i make that my multi-fasta file with the query sequence has a descriptions using the annotations hits that i get from blast, here is and example:

    >CL44Contig4 Nascent polypeptide associated complex alpha
    ACACGACGACTTCGAGCGGCCGAACCCGGGCAGGTACGAACGGGGGGCAAGCTA

    Anyone try to make this before???

  • #2

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    • #3
      hi
      could you be more specific? im starting with perl.

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      • #4
        please some help here.

        i have a big problem i just cant make with bioperl this;
        >CL56Contig1 Nascent polypeptide associated complex alpha
        MVGGGNRRDEASFTVTNTNGFAALESLRKKKKSDKEKGSSKGGKGSSKAAGGGQNAEPEK
        QVYLPGRQSSC..
        its seem to be rigth but the sequence is a protein and my query is a DNA :
        >CL56Contig1
        ACACGACGACTTCGAGCGGCCGAACCCGGGCAGGTACGAACGGGGGGCAAGCTA..

        how can i replace the protein secuence with the dna secuence? help me.

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        • #5
          Discussion of next-gen sequencing related bioinformatics: resources, algorithms, open source efforts, etc

          Comment

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