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Old 04-14-2010, 08:55 AM   #1
remcoloos
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Location: Cambridge, UK

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Default maximal fragment length GA?

Could anybody tell me what the maximal fragment length is at which the GA II amplification and sequencing would still work properly? And are there any specific precautions to be taken when working with longer fragments?

Specifically, we're looking at fragment of up to 700bp, would that be feasible?

Thanks in advance, Remco
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Old 04-14-2010, 03:20 PM   #2
gford
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Default Biotechniques reference

The linked paper describes 600 bp libraries prepared from PCR products:

http://www.biotechniques.com/Biotech...?autnID=187880
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