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Old 04-06-2017, 04:03 PM   #1
SDPA_Pet
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Default Can we sequence RNA directly

Hello, couple of years ago, I was in a NGS workshop. I was told some company was trying to develop some kits, so we can do metatranscriptomics without building cDNA libraries. I was wondering if this kit is available now?

Or now most sequencing center still use cDNA to do metatranscriptomics.
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Old 04-06-2017, 08:59 PM   #2
nucacidhunter
Jafar Jabbari
 
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I know two technologies for direct transcriptome analysis:
1- nanoString: it can only quantify known transcripts
2- MinIon: the kit currently is offered for early access

cDNA still is the most common method.
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Old 04-07-2017, 04:00 AM   #3
Bukowski
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http://biorxiv.org/content/early/2016/08/12/068809
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Old 04-07-2017, 04:17 AM   #4
gringer
David Eccles (gringer)
 
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Quote:
Originally Posted by nucacidhunter View Post
1- nanoString: it can only quantify known transcripts
nanoString doesn't "sequence" the RNA, unless you're referring to Hyb & Seq (which can sequence novel sequences, but is still in the technology development stages).
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