Hi Guys,
I was just wondering how you might deal with strand bias in DNA methylation calling using RRBS (reduced representation bisulphite sequencing). I know that strand bias is often an indication of erroneous alignment in NGS, so I am just not quite sure if I should filter out regions with disproportional number of plus and minus strands in estimating DNA methylation levels at CpG sites.
Thanks,
Hui
I was just wondering how you might deal with strand bias in DNA methylation calling using RRBS (reduced representation bisulphite sequencing). I know that strand bias is often an indication of erroneous alignment in NGS, so I am just not quite sure if I should filter out regions with disproportional number of plus and minus strands in estimating DNA methylation levels at CpG sites.
Thanks,
Hui
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