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  • Fixing BAM file header. Getting "truncated file. Aborting" error

    Hello.

    I have a BAM file that I need to filter and then update the header for.

    These are my commands:

    Code:
    samtools view -@ 32  -h -b -f 2 -T /data/hs37d5.fa \
      -o samp1234.clean.bam \
      bam.files/samp1234.bam \
      1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 X Y MT
    
    samtools view -H samp1234.clean.bam | \
      grep -v -e 'SN:GL' -e 'SN:NC' -e 'SN:hs37d5' \
      > samp1234.corrected.sam
    
    samtools reheader samp1234.corrected.sam \
      samp1234.clean.bam > samp1234.clean.reheaded.bam
    
    samtools sort -@ 32 \
      -o samp1234.clean.reheaded.sorted.bam \
      samp1234.clean.reheaded.bam

    The first 3 commands execute without any problems.
    Using
    Code:
    samtools view -h samp1234.clean.reheaded.bam | less -S
    I can see that the new BAM file does indeed contain the correct header.

    However every time I run the sorting command I get the error message:
    Code:
    samtools sort: truncated file. Aborting
    The samp1234.clean.reheaded.bam file seems to be fine in every regard. I can't seem to find a fault with it .

    Performing the reheading step in place doesn't seem to help nor does the -P option

    Does anyone have a suggestion of what I'm doing wrong?

    In case it helps I'm using samtools version 1.4-5-g692e058 (using htslib 1.4-5-g10bc1a7)

    Thanks in advance for any and all help.

  • #2
    Consider the answer given in this thread: https://www.biostars.org/p/289770/

    Comment


    • #3
      Thanks for pointing me to that post!
      I'm still having issues but I'll work with the suggested command there.

      Comment

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