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Old 04-20-2012, 01:03 AM   #1
Gorbenzer
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Default Alternative (cheaper) reagents for Ion Torrent PGM sequencer

Hi everyone,
in my lab we are trying to find a way for reduce the running costs of our new IonTorrent PGM sequencer... any suggestion?

Anyone found cheaper reagents that can substitute the ones sold by Life Technology?
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Old 05-09-2012, 01:49 AM   #2
jimmyinfrance
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We have checked out NEB kits, work OK (although still more expensive than I'd like).
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Old 05-14-2012, 09:28 PM   #3
genseq
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Genome Res. 2012 May;22(5):939-46. Epub 2012 Jan 20.
Cost-effective, high-throughput DNA sequencing libraries for multiplexed target capture.
http://www.ncbi.nlm.nih.gov/pubmed/22267522
"We present an approach in which 192 sequencing libraries can be produced in a single day of technician time at a cost of about $15 per sample."



How do SPRI beads work? (Ampure XP from Beckman)
http://core-genomics.blogspot.com/20...eads-work.html
“crowding agent” - polyethylene glycol (PEG) and salt (20% PEG, 2.5M NaCl is the magic mix)
Qiagen PE bottle contains 6ml of water




Reusable chips:
http://seqanswers.com/forums/showthread.php?t=19064

Last edited by genseq; 05-14-2012 at 10:33 PM. Reason: addition
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Old 06-04-2012, 07:16 AM   #4
genseq
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Cool

Cheap barcoding:
Attached Files
File Type: pdf Barcoding.pdf (315.0 KB, 282 views)
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Old 06-05-2012, 11:20 PM   #5
Gorbenzer
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Quote:
Originally Posted by genseq View Post
Cheap barcoding:
Looks really interesting, time to use google translator

Last edited by Gorbenzer; 06-05-2012 at 11:29 PM.
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Old 06-07-2012, 05:39 AM   #6
diagen
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Does anybody know the sequences of primers, adaptors and immobilized oligos?
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Old 08-25-2012, 09:37 AM   #7
genseq
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Cool

http://www.molecularecologist.com/20...re-substitute/
A penny for your method: AMPure Substitute
“[replacement for] a commonly used commercial kit (AMPure XP kit) for SPRI-clean-up steps with a home-made mix”
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Old 03-26-2013, 10:09 PM   #8
genseq
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Thumbs up New kit - $36...40 for 1 library (75 min)

Accel-NGS™ DNA Library Kit:
http://www.swiftbiosci.com/products/...na-library-kit
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File Type: pdf Swift Accel-NGS Press Release with Photo 2-27-13.pdf (170.3 KB, 48 views)

Last edited by genseq; 03-26-2013 at 10:21 PM. Reason: URL insertion
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Old 03-27-2013, 06:51 AM   #9
HeidiJTP
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genseq - thanks for the latest info!

How about the NEXTflex kits? Anyone had experience with their DNA-Seq kits or the barcoding?
http://www.biooscientific.com/Detail...A%20Sequencing
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Old 04-03-2013, 12:45 PM   #10
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Has anyone tried halving the AmpliSeq PCR reactions? I understand this would change the PCR dynamics...
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Old 11-30-2013, 04:20 PM   #11
salyamoff
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Quote:
Originally Posted by prooney View Post
Has anyone tried halving the AmpliSeq PCR reactions? I understand this would change the PCR dynamics...
This is intresting question. Nobody ever tried?

I did it with the cancer hotspot panel. Sequencing results are similar.

Last edited by salyamoff; 04-03-2014 at 11:23 AM.
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