Hi all,
In RNA-seq, after mapping (BOWTIE, unique hits only, max 2 mismatches), and annotating, we discovered that our reads generally fall in tall "stacks", as illustrated below:
Does anyone have a explanation for this? Could it be the sequencing step (or PCR bias), or could it be a artefact of the bioinformatic analysis?
Thanks in advance,
JW, Uni of Copenhagen
In RNA-seq, after mapping (BOWTIE, unique hits only, max 2 mismatches), and annotating, we discovered that our reads generally fall in tall "stacks", as illustrated below:
Does anyone have a explanation for this? Could it be the sequencing step (or PCR bias), or could it be a artefact of the bioinformatic analysis?
Thanks in advance,
JW, Uni of Copenhagen
Comment